Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD177 antibody [EPR22813-205] (ab255296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22813-205] to CD177
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD177 antibody [EPR22813-205]
    See all CD177 primary antibodies

  • Description

    Rabbit monoclonal [EPR22813-205] to CD177

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bone marrow. Human placenta and spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22813-205

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human bone marrow labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human bone marrow (PMID: 22434870). The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    All lanes : Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution

    Lane 1 : Human lung lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa



    CD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 secs; Lane 2: 3 mins.

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    All lanes : Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution

    Lane 1 : Human liver lysate
    Lane 2 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa


    Exposure time: 3 minutes


    CD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308). Negative control: U-937 (PMID: 17580308).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/5000 dilution + Recombinant his-tagged human CD177 protein (aa22-407), 20 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa


    Exposure time: 59 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in human spleen (PMID: 24562309) is observed. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on neutrophils in human placenta. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Anti-CD177 antibody [EPR22813-205] (ab255296)
    Anti-CD177 antibody [EPR22813-205] (ab255296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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