Anti-AKT1 antibody [1F7E10] (ab233755)
Key features and details
- Mouse monoclonal [1F7E10] to AKT1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-AKT1 antibody [1F7E10]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [1F7E10] to AKT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB HumanRecombinant fragment -
Immunogen
Recombinant fragment corresponding to Human AKT1 aa 1-150. Expressed in E.coli.
Database link: P31749 -
Positive control
- WB: Recombinant human AKT1 (aa 1-150); MCF7, NIH/3T3, COS-7 and C6 cell lysates; AKT1 (aa 1-150)-hIgGFc transfected HEK-293 whole cell lysate. ICC/IF: HepG2 cells. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1F7E10 -
Isotype
IgG1 -
Research areas
Images
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Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution + Recombinant human AKT1 (aa 1-150) protein
Developed using the ECL technique.
Predicted band size: 56 kDaExpected recombinant MW is 44 kDa.
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All lanes : Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : AKT1 (aa 1-150)-hIgGFc transfected HEK-293 whole cell lysate
Developed using the ECL technique.
Predicted band size: 56 kDa
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All lanes : Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 56 kDa
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AKT1 (green) using ab233755 in ICC/IF. Blue: DRAQ5 DNA dye. Red: Actin filaments stained with AlexaFluor®-555-Phalloidin.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AKT1 with ab233755 at 1/200 dilution (green) compared with a negative control (red).