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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT1 antibody [1F7E10] (ab233755)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-AKT1 antibody [1F7E10] (ab233755)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1F7E10] to AKT1
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-AKT1 antibody [1F7E10]
    See all AKT1 primary antibodies
  • Description

    Mouse monoclonal [1F7E10] to AKT1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human AKT1 aa 1-150. Expressed in E.coli.
    Database link: P31749

  • Positive control

    • WB: Recombinant human AKT1 (aa 1-150); MCF7, NIH/3T3, COS-7 and C6 cell lysates; AKT1 (aa 1-150)-hIgGFc transfected HEK-293 whole cell lysate. ICC/IF: HepG2 cells. Flow cyt: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1F7E10
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution + Recombinant human AKT1 (aa 1-150) protein

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    Expected recombinant MW is 44 kDa.

  • Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    All lanes : Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : AKT1 (aa 1-150)-hIgGFc transfected HEK-293 whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 56 kDa

  • Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    Western blot - Anti-AKT1 antibody [1F7E10] (ab233755)
    All lanes : Anti-AKT1 antibody [1F7E10] (ab233755) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 5 : C6 (rat glial tumor cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody [1F7E10] (ab233755)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody [1F7E10] (ab233755)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AKT1 (green) using ab233755 in ICC/IF. Blue: DRAQ5 DNA dye. Red: Actin filaments stained with AlexaFluor®-555-Phalloidin.

  • Flow Cytometry - Anti-AKT1 antibody [1F7E10] (ab233755)
    Flow Cytometry - Anti-AKT1 antibody [1F7E10] (ab233755)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AKT1 with ab233755 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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