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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Recombinant human AKT2 protein (ab79798)

Recombinant human AKT2 protein (ab79798)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

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Description

  • Product name

    Recombinant human AKT2 protein
    See all AKT2 proteins and peptides
  • Biological activity

    Specific Activity: 5.9 pmol/min/µg.
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P31751
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E
    • Amino acids

      1 to 481
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Recombinant full-length human AKT2 was co-expressed with PDK1 by baculovirus infection in Sf9 insect cells.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.1% Imidazole
    Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Western blot - Recombinant human AKT2 protein (ab79798)
    Western blot - Recombinant human AKT2 protein (ab79798)
    Anti-AKT2 antibody (ab78666) + Recombinant human AKT2 protein (ab79798) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  • SDS-PAGE - Recombinant human AKT2 protein (ab79798)
    SDS-PAGE - Recombinant human AKT2 protein (ab79798)

    10% SDS-PAGE showing ab79798 at approximately 57kDa (10µg).

  • Functional Studies - Recombinant human AKT2 protein (ab79798)
    Functional Studies - Recombinant human AKT2 protein (ab79798)
    Kinase assay: Specific activity 5.9 pmol/min/μg.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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