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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4157] to AKT1 (phospho T450) - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free
    See all AKT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4157] to AKT1 (phospho T450) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247614 is the carrier-free version of ab108266.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4157
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
    Western blot - Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
    All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] (ab108266) at 1/1000 dilution

    Lane 1 : NIH3T3 cell lysate, untreated
    Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108266, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
    This data was developed using ab108266, the same antibody clone in a different buffer formulation.ab108266 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)
    Anti-AKT1 (phospho T450) antibody [EPR4157] - BSA and Azide free (ab247614)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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