Anti-AKT1 antibody [AKT1/2552] (ab237994)
![Anti-AKT1 antibody [AKT1/2552] (ab237994)](https://www.abcam.com/ps/products/237/ab237994/Images/ab237994-343013-antiAKT1antibodyAKT12552-immunohistochemistry-human.jpg "Anti-AKT1 antibody [AKT1/2552] (ab237994)")
Key features and details
- Mouse monoclonal [AKT1/2552] to AKT1
- Suitable for: IHC-P, Protein Array, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-AKT1 antibody [AKT1/2552]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [AKT1/2552] to AKT1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Array, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human AKT1 aa 85-189. The exact sequence is proprietary.
Database link: P31749 -
Positive control
- IHC-P: Human pancreas tissue. WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Bioreactor Concentrate. -
Clonality
Monoclonal -
Clone number
AKT1/2552 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody [AKT1/2552] (ab237994)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for AKT1 using ab237994 at 2 µg/mL in immunohistochemical analysis.
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![Western blot - Anti-AKT1 antibody [AKT1/2552] (ab237994)](https://www.abcam.com/ps/products/237/ab237994/Images/ab237994-343012-antiAKT1antibodyAKT12552-western-blot-hela-human.jpg)
Western blot - Anti-AKT1 antibody [AKT1/2552] (ab237994)Anti-AKT1 antibody [AKT1/2552] (ab237994) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 55 kDa
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Protein Array - Anti-AKT1 antibody (ab237994)ab237994 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.