Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR121] to S100 alpha 6/PRA
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-S100 alpha 6/PRA antibody [EPNCIR121]
See all S100 alpha 6/PRA primary antibodies
Description

Rabbit monoclonal [EPNCIR121] to S100 alpha 6/PRA
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human S100 alpha 6/PRA.
Database link: P06703
Positive control
- Fetal lung, A549, ovary cancer, SH-SY5Y, and Human T-78 cell lysates, HeLa cells
General notes
This product was previously labelled as S100 alpha 6

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPNCIR121
Isotype

IgG
Research areas

Western blot - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

All lanes : Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149) at 1/1000 dilution

Lane 1 : Fetal lung cell lysates
Lane 2 : A549 cell lysates
Lane 3 : Ovary cancer cell lysates
Lane 4 : SH-SY5Y cell lysates
Lane 5 : Human T-78 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa
Flow Cytometry - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

Overlay histogram showing SHSY-5Y cells stained with ab134149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134149, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

Immunofluorescence analysis of HeLa cells labelled with ab134149 at 1/100 dilution.
Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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