Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)
![Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-212282-anti-s100-alpha-6-antibody-epr13084-69-western-blot.jpg "Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA
- Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-S100 alpha 6/PRA antibody [EPR13084-69]
See all S100 alpha 6/PRA primary antibodies -
Description
Rabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, Human fetal lung and A549 lysates; Human colonic carcinoma tissue; A549 cells; HeLa cells.
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General notes
This product was previously labelled as S100 alpha 6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13084-69 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)All lanes : Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal lung lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-353750-anti-s100-alpha-6-pra-antibody-epr1308469-immunocytochemistry-immunofluorescnece-a549-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling S100 alpha 6/PRA with ab181975 at 1/2000 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-212281-anti-s100-alpha-6-antibody-epr13084-69-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181975 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-212275-anti-s100-alpha-6-antibody-epr13084-69-flow-cytometry.jpg)
Flow Cytometry - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling S100 alpha 6/PRA with ab181975 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-212274-anti-s100-alpha-6-antibody-epr13084-69-immunoprecipitation.jpg)
Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)Western blot analysis of HeLa cell lysate immunoprecipitated with ab181975 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
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![Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)](https://www.abcam.com/ps/products/181/ab181975/Images/ab181975-8-benefits-of-recombinant-antibodies.png)
Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)
