Anti-AKT1 antibody (ab227385)
Key features and details
- Rabbit polyclonal to AKT1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 antibody
See all AKT1 primary antibodies -
Description
Rabbit polyclonal to AKT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Cat, Pig, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human AKT1 (internal sequence). The exact sequence is proprietary.
Database link: P31749 -
Positive control
- WB: AKT1 transfected HeLa whole cell lysate (ab150035). Mouse liver lysate. IP: AKT1 transfected HEK-293T whole cell lysate. IHC: Human lung adenocarcinoma tissue. ICC/IF: MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 antibody (ab227385)All lanes : Anti-AKT1 antibody (ab227385) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : AKT1 transfected HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody (ab227385)Paraffin-embedded human lung adenocarcinoma tissue stained for AKT1 using ab227385 at 1/250 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody (ab227385)Methanol fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for AKT1 (green) using ab227385 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-AKT1 antibody (ab227385)AKT1 was immunoprecipitated from AKT1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab227385. Western blot was performed from the immunoprecipitate using ab227385 at 1/5000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: AKT1 transfected HEK-293T whole cell lysate (Input).
Lane 2: Rabbit IgG instead of ab227385 in AKT1 transfected HEK-293T whole cell lysate.
Lane 2: ab227385 IP in AKT1 transfected HEK-293T whole cell lysate.
7.5% SDS PAGE
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Western blot - Anti-AKT1 antibody (ab227385)Anti-AKT1 antibody (ab227385) at 1/10000 dilution + Mouse liver tissue lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.7.5% SDS PAGE
