Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1B11] to LIM Kinase 1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free
    See all LIM Kinase 1 primary antibodies

  • Description

    Rat monoclonal [1B11] to LIM Kinase 1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255749 is the carrier-free version of ab243137. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)
    Western blot - Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)
    All lanes : Anti-LIM Kinase 1 antibody [1B11] (ab243137) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1 : 180 seconds; Lane 2 : 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243137).

  • Western blot - Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)
    Western blot - Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)
    All lanes : Anti-LIM Kinase 1 antibody [1B11] (ab243137) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse liver (PMID: 8537403).

    Exposure time: 81 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243137).

  • Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)
    Anti-LIM Kinase 1 antibody [1B11] - BSA and Azide free (ab255749)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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