Anti-RTR antibody (ab38816)
Key features and details
- Rabbit polyclonal to RTR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RTR antibody
See all RTR primary antibodies -
Description
Rabbit polyclonal to RTR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human RTR aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38815)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB (1) 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815).Notes WB
1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815).Target
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Function
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. -
Tissue specificity
Shows highest expression in the germ cells of the adult testis. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR6 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2649 Human
- Entrez Gene: 14536 Mouse
- Entrez Gene: 362125 Rat
- Omim: 602778 Human
- SwissProt: Q15406 Human
- SwissProt: Q64249 Mouse
- Unigene: 586460 Human
- Unigene: 439703 Mouse
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Alternative names
- GCNF 1 antibody
- GCNF antibody
- GCNF1 antibody
see all
Images
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Western blot - Anti-RTR antibody (ab38816)All lanes : Anti-RTR antibody (ab38816) at 1/250 dilution
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human testis tissue lysate - total protein (ab30257) withHuman RTR peptide (ab38815) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 230 kDa (possible cross reactivity) -
Western blot - Anti-RTR antibody (ab38816)This image is courtesy of an anonymous Abreview.All lanes : Anti-RTR antibody (ab38816) at 1/80 dilution
Lane 1 : Cell extract of HAmGCNF expressing COS-1 cells.
Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg
Secondary
All lanes : Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Datasheets and documents
References (4)
ab38816 has been referenced in 4 publications.
- Valdmanis PN et al. miR-122 removal in the liver activates imprinted microRNAs and enables more effective microRNA-mediated gene repression. Nat Commun 9:5321 (2018). PubMed: 30552326
- Chalmel F et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Hum Reprod 27:3233-48 (2012). IHC-P ; Human, Rat . PubMed: 22926843
- Bianco C et al. Regulation of human Cripto-1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells. J Cell Physiol : (2012). WB ; Human . PubMed: 23129342
- Zuccotti M et al. Oct-4 regulates the expression of Stella and Foxj2 at the Nanog locus: implications for the developmental competence of mouse oocytes. Hum Reprod 24:2225-37 (2009). ICC/IF ; Mouse . PubMed: 19477878
Images
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Western blot - Anti-RTR antibody (ab38816)All lanes : Anti-RTR antibody (ab38816) at 1/250 dilution
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human testis tissue lysate - total protein (ab30257) withHuman RTR peptide (ab38815) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 230 kDa (possible cross reactivity) -
Western blot - Anti-RTR antibody (ab38816) This image is courtesy of an anonymous Abreview.All lanes : Anti-RTR antibody (ab38816) at 1/80 dilution
Lane 1 : Cell extract of HAmGCNF expressing COS-1 cells.
Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg
Secondary
All lanes : Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
