Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free
    See all U2AF35/U2AF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12648(2)] to U2AF35/U2AF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251242 is the carrier-free version of ab197591 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251242 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as U2AF35

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/10000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab197591, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 1388271).

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    All lanes : Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/20000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab197591, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 1388271).

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Anti-U2AF35/U2AF1 antibody [EPR12648(2)] (ab197591) at 1/1000 dilution + Rat spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab197591, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 1388271).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)

    This data was developed using ab197591, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    This data was developed using ab197591, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U2AF35/U2AF1 with ab197591 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP)(ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Immunocytochemistry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    This data was developed using ab197591, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of RAMOS cells labelling U2AF35/U2AF1 with ab197591 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.
  • Flow Cytometry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Flow Cytometry - Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    This data was developed using ab197591, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos (Human Burkitt's lymphoma) cells labeling U2AF35/U2AF1 with ab197591 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)
    Anti-U2AF35/U2AF1 antibody [EPR12648(2)] - BSA and Azide free (ab251242)

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