Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)
![Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)](https://www.abcam.com/ps/products/272/ab272113/Images/ab272113-1-AKTpS473BiotinConjugatedMonoclonalAntibody1IHC.jpg "Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)")
Key features and details
- Biotin Mouse monoclonal [17F6.B11] to AKT1 (phospho S473)
- Suitable for: IHC-P, ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11]
See all AKT1 primary antibodies -
Description
Biotin Mouse monoclonal [17F6.B11] to AKT1 (phospho S473) -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
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Immunogen
Synthetic peptide within Human AKT1 (phospho S473). The exact sequence is proprietary. Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Database link: P31749 -
Positive control
- IHC-P: Human prostate tissue.
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General notes
10-20 moles Biotin per mole of IgG1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Buffer lyophilised from -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
17F6.B11 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)
Formalin-fixed, parafin embedded prostate )stained for AKT1 (red, left) using ab272113 at 20 µg/mL, followed by Streptavidin-HRP at 10 µg/mL. Counterstain: hematoxylin (purple). Negative control: right image.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)](https://www.abcam.com/ps/products/272/ab272113/Images/ab272113-2-AKTpS473BiotinConjugatedMonoclonalAntibody4IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)Formalin-fixed, paraffin embedded human prostate tissue stained for AKT1 (left) using ab272113 at 20 µg/mL, followed by Streptavidin-HRP at 10 µg/mL. Nuclear counterstain: hematoxylin. Antigen retrieval: Citrate, pH 6.2, pressure cooker.
Negative control (right) used pH9 antigen retrieval.
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![ELISA - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)](https://www.abcam.com/ps/products/272/ab272113/Images/ab272113-3-AKTpS473BiotinConjugatedMonoclonalAntibody3ELISA.jpg)
ELISA - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)ELISA of BSA conjugated Akt phospho S473 (red) and AKT non-phospho S473 (blue) at 0.1 µg/well using ab272113 at 5 µg/mL and 3-fold serial diluted, followed by Streptavidin-Peroxidase at 1/10,000. Substrate: TMB.