Astana Biomed Group, an authorized Abcam distributor in Central Asia

Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)

Key features and details

  • Biotin Mouse monoclonal [17F6.B11] to AKT1 (phospho S473)
  • Suitable for: IHC-P, ELISA
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11]
    See all AKT1 primary antibodies

  • Description

    Biotin Mouse monoclonal [17F6.B11] to AKT1 (phospho S473)

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: IHC-P, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee

  • Immunogen

    Synthetic peptide within Human AKT1 (phospho S473). The exact sequence is proprietary. Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
    Database link: P31749

  • Positive control

    • IHC-P: Human prostate tissue.

  • General notes

    10-20 moles Biotin per mole of IgG1

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)

    Formalin-fixed, parafin embedded prostate )stained for AKT1 (red, left) using ab272113 at 20 µg/mL, followed by Streptavidin-HRP at 10 µg/mL. Counterstain: hematoxylin (purple). Negative control: right image.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)

    Formalin-fixed, paraffin embedded human prostate tissue stained for AKT1 (left) using ab272113 at 20 µg/mL, followed by Streptavidin-HRP at 10 µg/mL. Nuclear counterstain: hematoxylin. Antigen retrieval: Citrate, pH 6.2, pressure cooker. 

    Negative control (right) used pH9 antigen retrieval.

  • ELISA - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)
    ELISA - Biotin Anti-AKT1 (phospho S473) antibody [17F6.B11] (ab272113)

    ELISA of BSA conjugated Akt phospho S473 (red) and AKT non-phospho S473 (blue) at 0.1 µg/well using ab272113 at 5 µg/mL and 3-fold serial diluted, followed by Streptavidin-Peroxidase at 1/10,000. Substrate: TMB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.