Human AKT1 knockout HeLa cell lysate (ab256835)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab261926 - Human AKT1 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human AKT1 knockout HeLa cell lysate (ab256835)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: AKT1 knockout HeLa cell lysate (20µg)
Lane 3: MCF7 cell lysate (20µg)
Lane 4: Daudi cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab108202 observed at 60 kDa. Red - loading control ab181602 observed at 37 kDa.
ab108202 Anti-AKT1 antibody [M1843] was shown to specifically react with AKT1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264852 (knockout cell lysate ab256835) was used. Wild-type and AKT1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108202 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human AKT1 knockout HeLa cell lysate (ab256835)Allele-1: 1 bp insertion in exon 2
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Sanger Sequencing - Human AKT1 knockout HeLa cell lysate (ab256835)Allele-2: Insertion of the selection cassette in exon 2
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Sanger Sequencing - Human AKT1 knockout HeLa cell lysate (ab256835)Allele-3: Insertion of the selection cassette in exon 2
