Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12352] to MGST3 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MGST3 antibody [EPR12352] - BSA and Azide free
    See all MGST3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12352] to MGST3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251124 is the carrier-free version of ab192254. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251124 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    All lanes : Anti-MGST3 antibody [EPR12352] (ab192254) at 1/10000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab192254, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)

    This data was developed using ab192254, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab192254, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + mouse spleen tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab192254, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    This data was developed using ab192254, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    This data was developed using ab192254, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Immunoprecipitation - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    This data was developed using ab192254, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)
    Anti-MGST3 antibody [EPR12352] - BSA and Azide free (ab251124)

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