Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GPA33 antibody [EPR4240] (ab108938)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4240] to GPA33
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-GPA33 antibody [EPR4240]
See all GPA33 primary antibodies
Description

Rabbit monoclonal [EPR4240] to GPA33
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human small intestine, colon and fetal colon tissue lysates; HT-29 and MOLT-4 cell lysates; IHC-P: Human colon tissue. Human colon cancer tissue. Human ovarian cancer tissue; IP: HT-29 cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4240
Isotype

IgG
Research areas

Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)

All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (Purified)

Lane 1 : Human colon lysates
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 36 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling GPA33 with Purified ab108938 at 1:400 dilution (0.25 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)

Purified ab108938 at 1:20 dilution (0.5ug) immunoprecipitating GPA33 in HT-29 whole cell lysate.

Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate (10µg)

Lane 2 (+): ab108938 + HT-29 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108938 in HT-29 whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.

Blocking Buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM/TBST.

Observed band size: 50 kDa
Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)

All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (unpurified)

Lane 1 : HT-29 lysate
Lane 2 : Human small intestine lysate
Lane 3 : Human fetal colon lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 showing negative staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 showing negative staining in Thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

Unpurified ab108938 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-GPA33 antibody [EPR4240] (ab108938)

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