Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GPA33 antibody [EPR4240] (ab108938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4240] to GPA33
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GPA33 antibody [EPR4240]
    See all GPA33 primary antibodies

  • Description

    Rabbit monoclonal [EPR4240] to GPA33

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine, colon and fetal colon tissue lysates; HT-29 and MOLT-4 cell lysates; IHC-P: Human colon tissue. Human colon cancer tissue. Human ovarian cancer tissue; IP: HT-29 cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4240

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
    Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
    All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (Purified)

    Lane 1 : Human colon lysates
    Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling GPA33 with Purified ab108938 at 1:400 dilution (0.25 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)

    Purified ab108938 at 1:20 dilution (0.5ug) immunoprecipitating GPA33 in HT-29 whole cell lysate.

    Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate (10µg)

    Lane 2 (+): ab108938 + HT-29 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108938 in HT-29 whole cell lysate.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.

    Blocking Buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    Observed band size: 50 kDa

  • Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
    Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
    All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (unpurified)

    Lane 1 : HT-29 lysate
    Lane 2 : Human small intestine lysate
    Lane 3 : Human fetal colon lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 showing negative staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 showing negative staining in Thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 showing negative staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

    Unpurified ab108938 showing negative staining in Normal liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GPA33 antibody [EPR4240] (ab108938)
    Anti-GPA33 antibody [EPR4240] (ab108938)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GPA33 antibody [EPR4240] (ab108938)

© 2021 Astana Biomed Group LLP. All rights reserved.