Anti-PTF1A antibody (ab125973)
Key features and details
- Rabbit polyclonal to PTF1A
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PTF1A antibody
See all PTF1A primary antibodies -
Description
Rabbit polyclonal to PTF1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
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Positive control
- This antibidy gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications Chinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).Target
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Function
Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development. -
Tissue specificity
Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers. -
Involvement in disease
Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069]. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex. - Information by UniProt
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Database links
- Entrez Gene: 19213 Mouse
- Entrez Gene: 117034 Rat
- SwissProt: Q9QX98 Mouse
- SwissProt: Q64305 Rat
- Unigene: 69647 Mouse
- Unigene: 10536 Rat
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Form
PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas. -
Alternative names
- bHLH transcription factor p48 antibody
- bHLHa29 antibody
- Class A basic helix-loop-helix protein 29 antibody
see all
Images
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Western blot - Anti-PTF1A antibody (ab125973)All lanes : Anti-PTF1A antibody (ab125973) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The predicted molecular weight of PTF1A is 35 kDa (SwissProt), however we expect to observe a banding pattern around 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125973 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab125973 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PTF1A antibody (ab125973)All lanes : Anti-PTF1A antibody (ab125973) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The predicted molecular weight of PTF1A is 35 kDa (SwissProt), however we expect to observe a banding pattern around 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125973 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
