Anti-PTF1A antibody [EPR19011] - BSA and Azide free (ab224794)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19011] to PTF1A - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PTF1A antibody [EPR19011] - BSA and Azide free
See all PTF1A primary antibodies -
Description
Rabbit monoclonal [EPR19011] to PTF1A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart and fetal kidney lysates; BxPC-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat heart and pancreas lysates; Mouse pancreas lysate. IP: NIH/3T3 whole cell lysate.
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General notes
Ab224794 is the carrier-free version of ab182398. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab224794 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19011 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab182398, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 18347078).
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All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution
Lane 1 : Rat heart tissue lysate at 20 µg
Lane 2 : Rat pancreas tissue lysate at 20 µg
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?This data was developed using ab182398, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lanes 3-6: 10 seconds.
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Anti-PTF1A antibody [EPR19011] (ab182398) at 1/5000 dilution + Mouse pancreas lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab182398, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab182398, the same antibody clone in a different buffer formulation.
PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).
Lane 2: ab182398 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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