Anti-MED9 antibody [OTI7F11] (ab236399)
![Anti-MED9 antibody [OTI7F11] (ab236399)](https://www.abcam.com/ps/products/236/ab236399/Images/ab236399-312480-3991.jpg "Anti-MED9 antibody [OTI7F11] (ab236399)")
Key features and details
- Mouse monoclonal [OTI7F11] to MED9
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MED9 antibody [OTI7F11]
See all MED9 primary antibodies -
Description
Mouse monoclonal [OTI7F11] to MED9 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MED9 aa 1 to the C-terminus. Produced in E.coli (NP_060489).
Database link: Q9NWA0 -
Positive control
- WB: pCMV6-ENTRY MED9 cDNA transfected HEK-293T cell lysate. IHC-P: Human breast and prostate carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI7F11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-MED9 antibody [OTI7F11] (ab236399)All lanes : Anti-MED9 antibody [OTI7F11] (ab236399) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY MED9 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 16 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (ab236399)](https://www.abcam.com/ps/products/236/ab236399/Images/ab236399-312482-3993.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (ab236399)Paraffin-embedded human prostate carcinoma tissue stained for MED9 using ab236399 at 1/1000 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (ab236399)](https://www.abcam.com/ps/products/236/ab236399/Images/ab236399-312481-3992.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (ab236399)Paraffin-embedded human breast tissue stained for MED9 using ab236399 at 1/1000 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
