Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nogo antibody [EPR12265] (ab180847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12265] to Nogo
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Nogo antibody [EPR12265]
    See all Nogo primary antibodies

  • Description

    Rabbit monoclonal [EPR12265] to Nogo

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Nogo aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NQC3

  • Positive control

    • U87-MG, SH-SY5Y and HeLa whole cell lysate (ab150035). Human brain and breast carcinoma tissues. U87-MG and HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nogo antibody [EPR12265] (ab180847)
    Western blot - Anti-Nogo antibody [EPR12265] (ab180847)
    All lanes : Anti-Nogo antibody [EPR12265] (ab180847) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 130 kDa
    Observed band size: 40-55 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human brain tissue labeling Nogo with ab180847 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human breast carcinoma tissue labeling Nogo with ab180847 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nogo antibody [EPR12265] (ab180847)
    Immunocytochemistry/ Immunofluorescence - Anti-Nogo antibody [EPR12265] (ab180847)

    Immunofluorescence analysis of U87-MG cells labeling Nogo with ab180847 at a 1/100 dilution.

  • Flow Cytometry - Anti-Nogo antibody [EPR12265] (ab180847)
    Flow Cytometry - Anti-Nogo antibody [EPR12265] (ab180847)

    Flow cytometry analysis of permeabilized HeLa cells labeling Nogo (red) with ab180847 at a 1/10 dilution, or negative control rabbit IgG (green)

  • Immunoprecipitation - Anti-Nogo antibody [EPR12265] (ab180847)
    Immunoprecipitation - Anti-Nogo antibody [EPR12265] (ab180847)

    Western blot analysis on Immunoprecipitation pellet from either 1) HeLa cell lysate, or 2) 1xPBS (negative control); showing Nogo, using ab180847 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-Nogo antibody [EPR12265] (ab180847)
    Anti-Nogo antibody [EPR12265] (ab180847)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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