Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Nogo antibody [EPR12265] (ab180847)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12265] to Nogo
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-Nogo antibody [EPR12265]
See all Nogo primary antibodies
Description

Rabbit monoclonal [EPR12265] to Nogo
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nogo aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NQC3
Positive control
- U87-MG, SH-SY5Y and HeLa whole cell lysate (ab150035). Human brain and breast carcinoma tissues. U87-MG and HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12265
Isotype

IgG
Research areas

Western blot - Anti-Nogo antibody [EPR12265] (ab180847)

All lanes : Anti-Nogo antibody [EPR12265] (ab180847) at 1/1000 dilution

Lane 1 : U87-MG cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 130 kDa
Observed band size: 40-55 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human brain tissue labeling Nogo with ab180847 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] (ab180847)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human breast carcinoma tissue labeling Nogo with ab180847 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Nogo antibody [EPR12265] (ab180847)

Immunofluorescence analysis of U87-MG cells labeling Nogo with ab180847 at a 1/100 dilution.
Flow Cytometry - Anti-Nogo antibody [EPR12265] (ab180847)

Flow cytometry analysis of permeabilized HeLa cells labeling Nogo (red) with ab180847 at a 1/10 dilution, or negative control rabbit IgG (green)
Immunoprecipitation - Anti-Nogo antibody [EPR12265] (ab180847)

Western blot analysis on Immunoprecipitation pellet from either 1) HeLa cell lysate, or 2) 1xPBS (negative control); showing Nogo, using ab180847 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-Nogo antibody [EPR12265] (ab180847)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com