Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)
![Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)](https://www.abcam.com/ps/products/251/ab251461/Images/ab251461-406101-anti-btg4-antibody-eprzju-21-bsa-and-azide-free-western-blot.jpg "Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRZJU-21] to BTG4 - BSA and Azide free
- Suitable for: WB, IP, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free
See all BTG4 primary antibodies -
Description
Rabbit monoclonal [EPRZJU-21] to BTG4 - BSA and Azide free -
Host species
Rabbit -
Specificity
We have not been able to detect the endogenous mouse BTG4 with this antibody.
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Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251461 is the carrier-free version of ab206914. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251461 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPRZJU-21 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
Lane 3 : Human ovary lysate
Lane 4 : Mouse ovary tissue lysate
Lane 5 : Mouse testis tissue lysate
Lane 6 : Rat ovary tissue lysate
Lane 7 : Rat testis tissue lysate
Lane 8 : Mouse P0 olfactory epithelium lysate
Lane 9 : Rat P0 olfactory epithelium lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab206914, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)](https://www.abcam.com/ps/products/251/ab251461/Images/ab251461-2-anti-btg4-antibody-eprzju-21-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)This data was developed using ab206914, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red). Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4. The nuclear counterstain is DAPI (blue). -
![Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)](https://www.abcam.com/ps/products/251/ab251461/Images/ab251461-1-anti-btg4-antibody-eprzju-21-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)This data was developed using ab206914, the same antibody clone in a different buffer formulation.BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input). Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second. -
![Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)](https://www.abcam.com/ps/products/251/ab251461/Images/ab251461-4-benefits-of-recombinant-antibodies.png)
Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (ab251461)