Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2452] to Tau (phospho S235) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR2452] to Tau (phospho S235) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody reacts with Tau only when phosphorilated at Serine 235.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab244235 is the carrier-free version of ab133253 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab244235 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    All lanes : Anti-Tau (phospho S235) antibody [EPR2452] (ab133253) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human hippocampus lysate - membrane incubated with phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody (HRP) at 1/2000 dilution

    Predicted band size: 78 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab133253, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    This data was developed using ab133253, the same antibody clone in a different buffer formulation.Dot blot analysis of Tau (pS235) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS235) with ab133253 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    All lanes : Anti-Tau (phospho S235) antibody [EPR2452] (ab133253) at 1/1000 dilution

    Lane 1 : SH SY5Y cell lysates
    Lane 2 : SH SY5Y cell lysates treated with Okadaic acid and Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa



    This data was developed using ab133253, the same antibody clone in a different buffer formulation.

    Beta tubulin is shown as loading control in the bottom panel.

  • Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
    Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)

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