Anti-Pin1 antibody [EP1479Y] (ab76309)
![Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.com/ps/products/76/ab76309/Images/ab76309-339725-ab76309WB1.jpg "Anti-Pin1 antibody [EP1479Y] (ab76309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1479Y] to Pin1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Pin1 antibody [EP1479Y]
See all Pin1 primary antibodies -
Description
Rabbit monoclonal [EP1479Y] to Pin1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
within Human Pin1 aa 50-150 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK-293, Mouse brain and Rat brain lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1479Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)All lanes : Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
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![Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.com/ps/products/76/ab76309/Images/ab76309-91699-anti-pin1-antibody-ep1479y-western-blot.jpg)
Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309)Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + HEK-293 cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
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![Anti-Pin1 antibody [EP1479Y] (ab76309)](https://www.abcam.com/ps/products/76/ab76309/Images/ab76309-5-benefits-of-recombinant-antibodies.png)
Anti-Pin1 antibody [EP1479Y] (ab76309)
