Anti-Pin1 antibody (ab12107)
Key features and details
- Rabbit polyclonal to Pin1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Pin1 antibody
See all Pin1 primary antibodies -
Description
Rabbit polyclonal to Pin1 -
Host species
Rabbit -
Specificity
ab12107 detects human Pin 1 at ~20kDa. ab12107 also detects a band at ~23kDa, which is not attenuated by blocking peptide ab12397; the identity of this band is unknown. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Pin1 aa 50-150 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab12397) -
Positive control
- This antibody gave a positive result with Pin1 recombinant protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa).Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa).Target
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Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5300 Human
- Entrez Gene: 23988 Mouse
- Entrez Gene: 298696 Rat
- Omim: 601052 Human
- SwissProt: Q13526 Human
- SwissProt: Q9QUR7 Mouse
- Unigene: 465849 Human
- Unigene: 7906 Mouse
see all -
Alternative names
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
Images
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Western blot - Anti-Pin1 antibody (ab12107)Lane 1 : Marker
Lanes 2-3 : Anti-Pin1 antibody (ab12107) at 1 µg/ml
Lane 2 : pin1 Recombinant 0.1ug
Lane 3 : pin1 Recombinant 0.5ug
Secondary
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18.2 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted?
The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).
Datasheets and documents
References (2)
ab12107 has been referenced in 2 publications.
- Bhaskaran N et al. Fbw7a and Fbw7? collaborate to shuttle cyclin E1 into the nucleolus for multiubiquitylation. Mol Cell Biol 33:85-97 (2013). Human . PubMed: 23109421
- Lv L et al. Inhibition of peptidyl-prolyl cis/trans isomerase Pin1 induces cell cycle arrest and apoptosis in vascular smooth muscle cells. Apoptosis 15:41-54 (2010). WB ; Human . PubMed: 19904610
Images
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Western blot - Anti-Pin1 antibody (ab12107)Lane 1 : Marker
Lanes 2-3 : Anti-Pin1 antibody (ab12107) at 1 µg/ml
Lane 2 : pin1 Recombinant 0.1ug
Lane 3 : pin1 Recombinant 0.5ug
Secondary
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18.2 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted?
The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).
