Anti-Smad2 antibody (ab228807)
Key features and details
- Rabbit polyclonal to Smad2
- Suitable for: WB, IP, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 antibody
See all Smad2 primary antibodies -
Description
Rabbit polyclonal to Smad2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey -
Immunogen
Recombinant fragment within Human Smad2 (internal sequence). The exact sequence is proprietary.
Database link: Q15796 -
Positive control
- IP: HeLa whole cell lysate. ChIP: HeLa whole cell lysate. ICC/IF: HeLa cells. WB: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Smad2 antibody (ab228807) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE gel.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (green) using ab228807 at 1/200 dilution in ICC/IF.
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ab228807 immunoprecipitates Smad2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of ab228807. The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.
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Smad2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab228807. Western blot was performed from the immunoprecipitate using ab228807 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell lysate 50 µg.
Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab228807 IP in HeLa whole cell lysate.