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Signal Transduction Signaling Pathway Nuclear Signaling SMADs

Anti-Smad2 antibody (ab228807)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Smad2 antibody (ab228807)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Smad2
  • Suitable for: WB, IP, ICC/IF, ChIP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Smad2 antibody
    See all Smad2 primary antibodies
  • Description

    Rabbit polyclonal to Smad2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, ChIPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Smad2 (internal sequence). The exact sequence is proprietary.
    Database link: Q15796

  • Positive control

    • IP: HeLa whole cell lysate. ChIP: HeLa whole cell lysate. ICC/IF: HeLa cells. WB: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Growth factors
    • TGF

Images

  • Western blot - Anti-Smad2 antibody (ab228807)
    Western blot - Anti-Smad2 antibody (ab228807)
    Anti-Smad2 antibody (ab228807) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 52 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab228807)
    Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab228807)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (green) using ab228807 at 1/200 dilution in ICC/IF.

  • ChIP - Anti-Smad2 antibody (ab228807)
    ChIP - Anti-Smad2 antibody (ab228807)

    ab228807 immunoprecipitates Smad2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of ab228807. The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.

  • Immunoprecipitation - Anti-Smad2 antibody (ab228807)
    Immunoprecipitation - Anti-Smad2 antibody (ab228807)

    Smad2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab228807. Western blot was performed from the immunoprecipitate using ab228807 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell lysate 50 µg.

    Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.

    Lane 3: ab228807 IP in HeLa whole cell lysate.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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