Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250)
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Smad2 (phospho S250) antibody [EPR2855(2)]
See all Smad2 primary antibodies -
Description
Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate treated with alkaline phosphatase; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2855(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557) at 1/1000 dilution
Lane 1 : untreated HeLa cells lysates
Lane 2 : HeLa cell lysate treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
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Flow cytometric analysis of 2% paraformaldehyde-fixed and permeabilized HeLa cells labeling Smad2 (phospho S250) with ab184557 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. HeLa cells labeled using ab184557 preincubated with Smad2 (phospho S250) peptide (blue) or non-phospho-Smad2 peptide (orange).
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