Anti-Smad2 (phospho S467) antibody (ab235265)
Key features and details
- Rabbit polyclonal to Smad2 (phospho S467)
- Suitable for: Dot blot, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 (phospho S467) antibody
See all Smad2 primary antibodies -
Description
Rabbit polyclonal to Smad2 (phospho S467) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human brain cortex. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody (ab235265)
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded human brain cortex tissue stained for Smad2 (phospho S467) using ab235265 at a 1/1000 dilution for 30 mins at RT, followed by a ready to use anti-rabbit poly HRP IgG for 8 mins at RT. Counterstained with hematoxylin.
HIER using citrate buffer for 20 mins was performed.
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Dot Blot - Anti-Smad2 (phospho S467) antibody (ab235265)Dot Blot - Anti-Smad2 (phospho S467) antibody (ab235265 at 1 µg/ml).
Lanes 1-5: 100, 33.33, 11.11, 3.70, 1.23 ng.
Row A: Smad2 phospho-S467 - BSA Peptide.
Row B: Smad2 S467 - BSA Peptide.
Row C: AKT1 pS473 - BSA Peptide.
Row D: AKT1 S473 - BSA Peptide.
Row E: Scramble Sprouty - BSA Peptide.
Row F: BSA.
Exposure time of 10 secs.
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Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S467) antibody (ab235265)Immunocytochemistry/ Immunofluorescence analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (phospho S467) using ab235265 at 15 µg/ml overnight at 4°C. A donkey anti-rabbit IgG DyLight™ 488 was used as the secondary, 5 µg/ml for 1 hour at RT.
