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Signal Transduction Signaling Pathway Nuclear Signaling SMADs

Anti-Smad1 (phospho S465) antibody (ab97689)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Smad1 (phospho S465) antibody (ab97689)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Smad1 (phospho S465)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Smad1 (phospho S465) antibody
    See all Smad1 primary antibodies
  • Description

    Rabbit polyclonal to Smad1 (phospho S465)
  • Host species

    Rabbit
  • Specificity

    ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465. The region surrounding Smad1 phospho S465 is highly conserved in several other Smad proteins, including Smad 5 and Smad 9. Cross reactivity with other Smad proteins is likely.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

  • Positive control

    • HeLa cell extracts treated with PMA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab97689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Growth factors
    • TGF

Images

  • Western blot - Anti-Smad1 (phospho S465) antibody (ab97689)
    Western blot - Anti-Smad1 (phospho S465) antibody (ab97689)
    All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilution

    Lane 1 : HeLa cell extracts, untreated
    Lane 2 : HeLa cell extracts, treated with PMA

    Predicted band size: 52 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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