Anti-Smad1 (phospho S465) antibody (ab97689)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S465)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad1 (phospho S465) antibody
See all Smad1 primary antibodies -
Description
Rabbit polyclonal to Smad1 (phospho S465) -
Host species
Rabbit -
Specificity
ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465. The region surrounding Smad1 phospho S465 is highly conserved in several other Smad proteins, including Smad 5 and Smad 9. Cross reactivity with other Smad proteins is likely. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).
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Positive control
- HeLa cell extracts treated with PMA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab97689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas