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Signal Transduction Signaling Pathway Nuclear Signaling SMADs

Anti-Smad1 antibody (ab131550)

Anti-Smad1 antibody (ab131550)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Smad1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Smad1 antibody
    See all Smad1 primary antibodies
  • Description

    Rabbit polyclonal to Smad1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region around C terminal amino acids 461-465 (

    ISSVS

    ) of Human Smad1 (NP_001003688.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; HeLa cell extract

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Growth factors
    • TGF

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody (ab131550)
    Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody (ab131550)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling Smad1 with ab131550 at 1/100 dilution.
  • Western blot - Anti-Smad1 antibody (ab131550)
    Western blot - Anti-Smad1 antibody (ab131550)
    Anti-Smad1 antibody (ab131550) at 1/500 dilution + HeLa cell extract

    Predicted band size: 52 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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