Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smad1 antibody [EPR5522] (ab126761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5522] to Smad1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Smad1 antibody [EPR5522]
    See all Smad1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5522] to Smad1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Smad1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HT1080, HUVEC, A673, HepG2, and 293T cell lysates. IHC-P: Human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Smad1 antibody [EPR5522] (ab126761)
    Western blot - Anti-Smad1 antibody [EPR5522] (ab126761)
    All lanes : Anti-Smad1 antibody [EPR5522] (ab126761) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SMAD1 knockout HeLa cell lysate
    Lane 3 : HT1080 cell lysate
    Lane 4 : Huvec cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Lanes 1- 4: Merged signal (red and green). Green - ab126761 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab126761 was shown to react with Smad1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265400 (knockout cell lysate ab257686) was used. Wild-type HeLa and SMAD1 knockout HeLa cell lysates were subjected to SDS-PAGE. ab126761 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 antibody [EPR5522] (ab126761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 antibody [EPR5522] (ab126761)

    ab126761, at 1/50 dilution, staining Smad1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Smad1 antibody [EPR5522] (ab126761)
    Western blot - Anti-Smad1 antibody [EPR5522] (ab126761)
    All lanes : Anti-Smad1 antibody [EPR5522] (ab126761) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A673 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • OI-RD Scanning - Anti-Smad1 antibody [EPR5522] (ab126761)
    OI-RD Scanning - Anti-Smad1 antibody [EPR5522] (ab126761)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Smad1 antibody [EPR5522] (ab126761)
    Anti-Smad1 antibody [EPR5522] (ab126761)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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