Anti-Smad4 antibody (ab244370)
Key features and details
- Rabbit polyclonal to Smad4
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Smad4 antibody
See all Smad4 primary antibodies -
Description
Rabbit polyclonal to Smad4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human Smad4 aa 100-250.
Database link: Q13485 -
Positive control
- IHC-P: Human testis, endometrium, and placenta tissues; WB: SCLC-21H and NIH/3T3 cell lysate; ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (ab244370)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling Smad4 with ab244370 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-Smad4 antibody (ab244370) -
Western blot - Anti-Smad4 antibody (ab244370)Anti-Smad4 antibody (ab244370) at 0.04 µg/ml + NIH-3T3 mouse cell line
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (ab244370)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling Smad4 with ab244370 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (ab244370)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Smad4 with ab244370 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-Smad4 antibody (ab244370)Anti-Smad4 antibody (ab244370) at 0.4 µg/ml + SCLC-21H cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody (ab244370)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Smad4 with ab244370 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
