Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP306] to Smad4 - BSA and Azide free
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P
Reacts with: Rat, Human

Product name

Anti-Smad4 antibody [SP306] - BSA and Azide free
See all Smad4 primary antibodies
Description

Rabbit monoclonal [SP306] to Smad4 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, IHC-Pmore details
Species reactivity

Reacts with: Rat, Human
Predicted to work with: Mouse
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow Cyt (intra): NIH/3T3, HepG2, and C6 cells. ICC/IF: NIH/3T3, HepG2, and C6 cells. IHC-P: Human placenta, pancreas, pancreatic adenocarcinoma, kidney, renal cell carcinoma, colon and colon adenocarcinoma tissues.
General notes
ab243929 is the carrier-free version of ab217267.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP306
Isotype

IgG
Research areas

Flow Cytometry - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93741).
Flow Cytometry - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Flow Cytometry analysis of NIH/3T3( Mouse embryonic fibroblast) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217267).
Flow Cytometry - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Flow Cytometry analysis of HepG2(Human hepatocellular carcinoma epithelial cell) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217267).
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93741).
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217267).
Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Flow Cytometry - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Flow cytometric analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Smad4 with ab217267 at 1/400 dilution for 30 minutes at 4°C (green), compared to a negative control cell of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab217267).
Anti-Smad4 antibody [SP306] - BSA and Azide free (ab243929)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com