Anti-MADH7/SMAD7 antibody (ab216428)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-MADH7/SMAD7 antibody
See all MADH7/SMAD7 primary antibodies -
Description
Rabbit polyclonal to MADH7/SMAD7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide within Human MADH7/SMAD7 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O15105 -
Positive control
- IHC-P: Rat kidney and lung tissue. WB: Mouse lung, lymph, spleen, stomach, large intestine and placenta lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse placenta tissue lysate
Lane 3 : Mouse large intestine tissue lysate
Secondary
All lanes : Conjugated secondary antibody at 1/20000 dilution
Predicted band size: 46 kDaPrimary incubation: 4°C overnight followed by conjugated secondary antibody incubation for 60 min at 37°C.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling MADH7/SMAD7 with ab216428 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + mouse lymph node at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse spleen lysate at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 46 kDa
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Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse stomach lysate at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling MADH7/SMAD7 with ab216428 at 1/600 dilution followed by conjugation to the secondary antibody for 20 minutes and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min. Endogenous peroxidase was blocked by 3% hydrogen peroxide for 20 minutes and blocking buffer (normal goat serum) added at 37°C for 30min.