Anti-Smad1 (phospho S187) antibody (ab73211)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S187)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Smad1 (phospho S187) antibody
See all Smad1 primary antibodies -
Description
Rabbit polyclonal to Smad1 (phospho S187) -
Host species
Rabbit -
Specificity
ab73211 detects endogenous levels of Smad1 only when phosphorylated at serine 187. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of serine 187 (PHSPPN)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad1 (phospho S187) antibody (ab73211)All lanes : Anti-Smad1 (phospho S187) antibody (ab73211) at 1/500 dilution
Lane 1 : Mouse muscle cell extract
Lane 2 : Mouse muscle cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 (phospho S187) antibody (ab73211)Immunohistochemical analysis of Smad1 in paraffin-embedded human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
