Anti-MADH7/SMAD7 antibody (ab272928)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MADH7/SMAD7 antibody
See all MADH7/SMAD7 primary antibodies -
Description
Rabbit polyclonal to MADH7/SMAD7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MADH7/SMAD7 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O15105 -
Positive control
- IHC-Fr: Mouse cerebellum tissue. ICC/IF: Rat E18 primary cortical neuron cells. WB: DDDDK-tagged MADH7 transfected HEK-293T whole cell extract. U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-MADH7/SMAD7 antibody (ab272928)
Frozen sections of mouse cerebellum tissue stained for MADH7/SMAD7 using ab272928 at 1/250 dilution in immunohistochemical analysis (green). Red: NF-H. Blue: DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-MADH7/SMAD7 antibody (ab272928)4% PFA-fixed rat E18 primary cortical neuron cells stained for MADH7/SMAD7 using ab272928 at 1/500 dilution in ICC/IF (green). Red: Tau. Blue: DAPI.
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Western blot - Anti-MADH7/SMAD7 antibody (ab272928)All lanes : Anti-MADH7/SMAD7 antibody (ab272928) at 1/5000 dilution
Lane 1 : Mock transfected HEK-293T cell lysate
Lane 2 : MAD1-transfected HEK-293T cell lysate
Lane 3 : SMAD2 transfected HEK-293T cell lysate
Lane 4 : SMAD3 transfected HEK-293T cell lysate
Lane 5 : SMAD4 transfected HEK-293T cell lysate
Lane 6 : SMAD5 transfected HEK-293T cell lysate
Lane 7 : SMAD7 transfected HEK-293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-MADH7/SMAD7 antibody (ab272928)All lanes : Anti-MADH7/SMAD7 antibody (ab272928) at 1/2000 dilution
Lane 1 : U-87 MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE