Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
![Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-91671-anti-smad5-phospho-s463-s465-antibody-ep7282ay-western-blot.jpg "Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]
See all SMAD5 primary antibodies -
Description
Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465) -
Host species
Rabbit -
Specificity
Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.
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Positive control
- HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP728(2)AY -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with BMP-4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-91670-anti-smad5-phospho-s463-s465-antibody-ep7282ay-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution. -
![Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)](https://www.abcam.com/ps/products/76/ab76296/Images/ab76296-3-benefits-of-recombinant-antibodies.png)
Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)
