Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Friday March 05, 2021

Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]
See all SMAD5 primary antibodies
Description

Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)
Host species

Rabbit
Specificity

Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.
Positive control
- HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP728(2)AY
Isotype

IgG
Research areas

Western blot - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)

All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution

Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with BMP-4

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)

Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution.
Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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