Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MMC-1-104-3] to SMAD5 (phospho S463 + S465) - BSA and Azide free
  • Suitable for: IHC-P, WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free
    See all SMAD5 primary antibodies

  • Description

    Rabbit monoclonal [MMC-1-104-3] to SMAD5 (phospho S463 + S465) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Dot blotmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SMAD5. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate IHC-P: Human breast carcinoma tissue and Human colonic carcinoma tissue

  • General notes

    Ab168252 is the carrier-free version of ab92698. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab168252 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SMAD5 (phospho S463 + S465) with purified ab92698 at a dilution of 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling SMAD5 (phospho S463 + S465) with purified ab92698 at a dilution of 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SMAD5 (phospho S463 + S465) with purified ab92698 at a dilution of 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Dot Blot - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Dot Blot - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Dot blot analysis of SMAD5 (pS463 + pS465) peptide (Lane 1), SMAD5 (pS465) peptide (Lane 2), SMAD5 (pS463) peptide (Lane 3) and SMAD5 non-phospho peptide (Lane 4) labelling SMAD5 (pS465) with purified ab92698 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (1) human breast carcinoma and (2) human colonic carcinoma tissues labelling SMAD5 (phospho S463 + P465) with unpurified ab92698 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling SMAD5 (phospho S463 + S465) with unpurified ab92698.
    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling SMAD5 (phospho S463 + S465) with unpurified ab92698.
    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SMAD5 (phospho S463 + S465) with unpurified ab92698.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling SMAD5 (phospho S463 + S465) with unpurified ab92698.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92698).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)
    Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - BSA and Azide free (ab168252)

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