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Signal Transduction Signaling Pathway Nuclear Signaling SMADs

Anti-Smad3 (phospho T179) antibody (ab74062)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Smad3 (phospho T179) antibody (ab74062)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Smad3 (phospho T179)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Smad3 (phospho T179) antibody
    See all Smad3 primary antibodies
  • Description

    Rabbit polyclonal to Smad3 (phospho T179)
  • Host species

    Rabbit
  • Specificity

    ab74062 detects endogenous levels of Smad3 only when phosphorylated at threonine 179.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).

  • Positive control

    • HeLa cell extracts treated with TNF (20ng/ml, 2mins); Human thymus gland tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Frozen Stock (-20C). Shelf life 12 months.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab74062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • SMAD family
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Smad3 (phospho T179) antibody (ab74062)
    Western blot - Anti-Smad3 (phospho T179) antibody (ab74062)
    All lanes : Anti-Smad3 (phospho T179) antibody (ab74062) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated
    with TNF (20ng/ml, 2mins)
    Lane 2 : HeLa cell extracts treated
    with TNF (20ng/ml, 2mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (ab74062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho T179) antibody (ab74062)
    ab74062 at 1/50 dilution staining Smad3 in human thymus gland by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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