Anti-Smad1 (phospho S206) antibody (ab106093)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S206)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad1 (phospho S206) antibody
See all Smad1 primary antibodies -
Description
Rabbit polyclonal to Smad1 (phospho S206) -
Host species
Rabbit -
Specificity
ab106093 is specific for Human Smad1 phosphorylated at pS206. Reactivity with nonphosphorylated Human Smad1 is minimal by ELISA and western blot. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Macaque monkey, Gorilla, Opossum, Orangutan
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Immunogen
A phosphorylated synthetic peptide corresponding to amino acids surrounding serine 206 of Human Smad1.
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Positive control
- HEPG2 whole cell lysates treated with BMP2, EGF, or NaCl.
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General notes
Expiration date is one year from date of opening.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab1060093 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad1 (phospho S206) antibody (ab106093)All lanes : Anti-Smad1 (phospho S206) antibody (ab106093) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate, treated with PBS
Lanes 2-3 : HepG2 whole cell lysate, treated with BMP2 (5 ng/ml)
Lane 4 : HepG2 whole cell lysate, treated with NaCL
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated a-Rabbit IgG at 1/3000 dilution
Predicted band size: 52 kDa
Cell lysates were incubated for 1 h at 37°C.
Primary antibody was pre-incubated before reacting with blot as follows:
top row - with PBS,
middle row - with the immunizing phosphorylated peptide
bottom row - with control or non-phosphorylated peptide.
