Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD-L2 antibody [CAL28] (ab256386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL28] to PD-L2
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PD-L2 antibody [CAL28]
    See all PD-L2 primary antibodies

  • Description

    Rabbit monoclonal [CAL28] to PD-L2

  • Host species

    Rabbit

  • Specificity

    Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HDLM-2, MDA-MB-231, U-2 OS, MG-63 and Saos-2 whole cell lysates; human tonsil, lung and lung cancer tissue lysates. IP: HDLM-2 whole cell lysate. Flow cyt: HDLM-2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    CAL28

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)
    Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)
    All lanes : Anti-PD-L2 antibody [CAL28] (ab256386) at 1/1000 dilution

    Lane 1 : MDLM-2 (human Hodgkin lymphoma) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 45-60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Negative control: Jurkat. (PMID: 27631416).

  • Flow Cytometry - Anti-PD-L2 antibody [CAL28] (ab256386)
    Flow Cytometry - Anti-PD-L2 antibody [CAL28] (ab256386)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HDLM-2 (human Hodgkin lymphoma) cells labeling PD-L2 with ab256386 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (ab256386)
    Immunoprecipitation - Anti-PD-L2 antibody [CAL28] (ab256386)

    PD-L2 was immunoprecipitated from 0.35 mg of HDLM-2 (human Hodgkin lymphoma) whole cell lysate with ab256386 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab256386X at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.


    Lane 1: HDLM-2 whole cell lysate 10 μ (Input).
    Lane 2: ab256386 IP in HDML-2 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256386 in HDML-2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

  • Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)
    Western blot - Anti-PD-L2 antibody [CAL28] (ab256386)
    All lanes : Anti-PD-L2 antibody [CAL28] (ab256386) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 3 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
    Lane 4 : Saos-2 (human osteosarcoma cell line) whole cell lysate
    Lane 5 : Human tonsil lysate
    Lane 6 : Human lung lysate
    Lane 7 : Human lung cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 45-60 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-PD-L2 antibody [CAL28] (ab256386)
    Anti-PD-L2 antibody [CAL28] (ab256386)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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