Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)
![Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)](https://www.abcam.com/ps/products/261/ab261838/Images/ab261838-356234-anti-pd-l2-antibody-cal28-flow-cytometry-human.jpg "Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL28] to PD-L2 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-PD-L2 antibody [CAL28] - BSA and Azide free
See all PD-L2 primary antibodies -
Description
Rabbit monoclonal [CAL28] to PD-L2 - BSA and Azide free -
Host species
Rabbit -
Specificity
Although this clone performed well in IHC-P on tonsil tissue, non-specific nuclear staining was seen on TMA. The staining pattern did not correlate with ISH, so we cannot recommend this clone for IHC.
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Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HDLM-2, MDA-MB-231, U-2 OS, MG-63 and Saos-2 whole cell lysates; human tonsil, lung and lung cancer tissue lysates. IP: HDLM-2 whole cell lysate. Flow cyt: HDLM-2 cells.
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General notes
ab261838 is the carrier-free version of ab256386. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab261838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CAL28 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HDLM-2 (human Hodgkin lymphoma) cells labeling PD-L2 with ab256386 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab256386).
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![Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)](https://www.abcam.com/ps/products/261/ab261838/Images/ab261838-356236-anti-pd-l2-antibody-cal28-immunoprecipitation-human.jpg)
Immunoprecipitation - Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)PD-L2 was immunoprecipitated from 0.35 mg of HDLM-2 (human Hodgkin lymphoma) whole cell lysate with ab256386 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab256386X at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab256386).
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![Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)](https://www.abcam.com/ps/products/261/ab261838/Images/ab261838-7-benefits-of-recombinant-antibodies.png)
Anti-PD-L2 antibody [CAL28] - BSA and Azide free (ab261838)
