Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)
Key features and details
- Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMAD1/3/5 (phospho S423 + S425) antibody -
Description
Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425) -
Host species
Rabbit -
Specificity
ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human SMAD1/3/5 aa 417-425.
Sequence:PSIRCSSVS
Database link: P84022 -
Positive control
- WB: HEK293, HeLa, MCF-7, Jurkat, A431, A549, LNCap, MOLT-4, Ramos, Raji, A-172, NIH/3T3 Lysate; MEF lysate; Hep3B lysat.
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General notes
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.33% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51451 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/ml
Lane 1 : HEK293 lysate
Lane 2 : HeLa Lysate
Lane 3 : MCF-7 Lysate
Lane 4 : Jurkat Lysate
Lane 5 : A431 Lysate
Lane 6 : A549 Lysate
Lane 7 : LNCap Lysate
Lane 8 : MOLT-4 Lysate
Lane 9 : Ramos Lysate
Lane 10 : Raji Lysate
Lane 11 : A-172 Lysate
Lane 12 : NIH/3T3 Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-12 : Peroxidase rabbit secondary antibody at 1/30000 dilution
Lane 3 : Peroxidase rabbit secondary antibody
Predicted band size: 48 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
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Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution
Lane 1 : MEF lysate (control)
Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour
Lane 3 : MEF-/-Smad3 lysate (control)
Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 5 : Hep3B lysate (control)
Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 7 : HEK 293 lysate (control)
Predicted band size: 48 kDa
Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.
