Anti-Smad4 antibody (ab236321)
Key features and details
- Rabbit polyclonal to Smad4
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Smad4 antibody
See all Smad4 primary antibodies -
Description
Rabbit polyclonal to Smad4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Smad4 aa 1-200.
Database link: Q13485 -
Positive control
- WB: SH-SY5Y, 3T3 and HepG2 whole cell lysate. IHC-P: Human colon cancer and kidney tissue. IP: Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Protein >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Smad4 antibody (ab236321) at 1/500 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60 kDa
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Paraffin-embedded human kidney tissue stained for Smad4 using ab236321 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon cancer tissue stained for Smad4 using ab236321 at 1/100 dilution in immunohistochemical analysis.
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Anti-Smad4 antibody (ab236321) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60 kDa
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Smad4 was immunoprecipitated from 0.5 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab236321.
Lane 1: Rabbit control IgG instead of ab236321 in Jurkat whole cell lysate.
Lane 2: ab236321 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 10 µg (Input).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.