Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP306] to Smad4 - C-terminal
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Smad4 antibody [SP306] - C-terminal
    See all Smad4 primary antibodies

  • Description

    Rabbit monoclonal [SP306] to Smad4 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Rat
    Human
    ICC/IF
    Mouse
    Rat
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: NIH/3T3, HepG2, and C6 cells. ICC/IF: NIH/3T3, HepG2, and C6 cells. IHC-P: Human placenta, pancreas, pancreatic adenocarcinoma, kidney, renal cell carcinoma, colon and colon adenocarcinoma tissues.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry analysis of NIH/3T3( Mouse embryonic fibroblast) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry analysis of HepG2(Human hepatocellular carcinoma epithelial cell) cells labeling Smad4 with purified ab217267 at 1:450 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Smad4 with purified ab217267 at 1:50 (9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Flow Cytometry - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Flow cytometric analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Smad4 with ab217267 at 1/400 dilution for 30 minutes at 4° C (green), compared to a negative control cell of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue labeling Smad4 with ab217267 at 1/100 dilution.

  • Anti-Smad4 antibody [SP306] - C-terminal (ab217267)
    Anti-Smad4 antibody [SP306] - C-terminal (ab217267)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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