Anti-Smad3 (phospho S425) antibody (ab51177)
Key features and details
- Rabbit polyclonal to Smad3 (phospho S425)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad3 (phospho S425) antibody
See all Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad3 (phospho S425) -
Host species
Rabbit -
Specificity
ab51177 detects endogenous levels of Smad3 only when phosphorylated at serine 425. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad3 (phospho S425) antibody (ab51177)Ab47439, at dilution of 1/50, staining Smad3 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: No peptide.
Right image: Phosphopeptide present.
