Anti-Smad3 (phospho T179) antibody (ab193297)
Key features and details
- Rabbit polyclonal to Smad3 (phospho T179)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad3 (phospho T179) antibody
See all Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad3 (phospho T179) -
Host species
Rabbit -
Specificity
ab193297 detects endogenous level of Smad3 only when phosphorylated at threonine 179. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Smad3 (phospho T179) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. [Peptide sequence around phosphorylation site of threonine179(
P-E-T(p)-P-P
).]
Database link: P84022 -
Positive control
- Extracts from HeLa cells treated with TNF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193297 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad3 (phospho T179) antibody (ab193297)All lanes : Anti-Smad3 (phospho T179) antibody (ab193297) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated with TNF
Lane 2 : extracts from HeLa cells treated with TNF with antigen-specific peptide
Predicted band size: 48 kDa
