Anti-Smad2 antibody (ab228765)
Key features and details
- Rabbit polyclonal to Smad2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 antibody
See all Smad2 primary antibodies -
Description
Rabbit polyclonal to Smad2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human Smad2 (internal sequence). The exact sequence is proprietary.
Database link: Q15796 -
Positive control
- WB: Jurkat whole cell lysate. IHC-P: Mouse liver and rat testis tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad2 antibody (ab228765)Anti-Smad2 antibody (ab228765) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 52 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab228765)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (green) using ab228765 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (ab228765)Paraffin-embedded mouse liver tissue stained for Smad2 using ab228765 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (ab228765)Paraffin-embedded rat testis tissue stained for Smad2 using ab228765 at 1/500 dilution in immunohistochemical analysis.
