Anti-Lingo1 antibody (ab23631)
Key features and details
- Rabbit polyclonal to Lingo1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Lingo1 antibody
See all Lingo1 primary antibodies -
Description
Rabbit polyclonal to Lingo1 -
Host species
Rabbit -
Specificity
Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modification and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4) -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide corresponding to Human Lingo1 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab25890)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab23631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). ICC/IF Use a concentration of 5 µg/ml. IP Use at an assay dependent concentration. Target
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Function
Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. -
Tissue specificity
Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsN-glycosylated. Contains predominantly high-mannose glycans. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 415344 Chicken
- Entrez Gene: 84894 Human
- Entrez Gene: 235402 Mouse
- Entrez Gene: 315691 Rat
- Omim: 609791 Human
- SwissProt: Q50L44 Chicken
- SwissProt: Q96FE5 Human
- SwissProt: Q9D1T0 Mouse
see all -
Alternative names
- FLJ14594 antibody
- LERN 1 antibody
- LERN1 antibody
see all
Images
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Western blot - Anti-Lingo1 antibody (ab23631)All lanes : Anti-Lingo1 antibody (ab23631) at 1 µg/ml
Lane 1 : Mouse brain
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Human Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 17 kDa (possible cleavage fragment), 34 kDa (possible degradation product)Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)
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Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)ICC/IF image of ab23631 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-Lingo1 antibody (ab23631)Lingo1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Lingo1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23631.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 83kDa:Lingo1; non specific - 70kDa: We are unsure as to the identity of this extra band. -
Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)ICC/IF image of ab23631 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)Images cropped from Lööv et al., PLoS One, 1, e29771, Fig. 1.; DOI: 10.1371/journal.pone.0029771 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemical analysis of mouse neural stem and progenitor cells, labeling Lingo1 with ab23631. Cells were fixed in 4% paraformaldehyde, then permeabilized and blocked for 30 minutes in 5% natural goat serum in PBS. Incubation with ab23631 (diluted 1/200) was for 1-4 hours at RT. DAPI was used for staining nuclei.
Top Left: Lingo1 staining
Top Right: Nestin staining
Bottom Left: Merge
Protocols
References (16)
ab23631 has been referenced in 16 publications.
- Quan Y et al. Inhibition of the leucine-rich repeat protein lingo-1 enhances RGC survival in optic nerve injury. Exp Ther Med 19:619-629 (2020). PubMed: 31885701
- Han KH et al. Axonal sprouting in the dorsal cochlear nucleus affects gap-prepulse inhibition following noise exposure. Int J Mol Med 44:1473-1483 (2019). PubMed: 31432095
- He R et al. Effect of fasudil on cognitive function following status convulsion in rats. Mol Med Rep 16:119-126 (2017). WB ; Rat . PubMed: 28534935
- Theotokis P et al. Nogo receptor complex expression dynamics in the inflammatory foci of central nervous system experimental autoimmune demyelination. J Neuroinflammation 13:265 (2016). PubMed: 27724971
- Fernandez-Enright F et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014). WB ; Human . PubMed: 24448210
Images
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Western blot - Anti-Lingo1 antibody (ab23631)All lanes : Anti-Lingo1 antibody (ab23631) at 1 µg/ml
Lane 1 : Mouse brain
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Human Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 17 kDa (possible cleavage fragment), 34 kDa (possible degradation product)Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)
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Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)
ICC/IF image of ab23631 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-Lingo1 antibody (ab23631)Lingo1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Lingo1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23631.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 83kDa:Lingo1; non specific - 70kDa: We are unsure as to the identity of this extra band. -
Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)
ICC/IF image of ab23631 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631) Images cropped from Lööv et al., PLoS One, 1, e29771, Fig. 1.; DOI: 10.1371/journal.pone.0029771 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunocytochemical analysis of mouse neural stem and progenitor cells, labeling Lingo1 with ab23631. Cells were fixed in 4% paraformaldehyde, then permeabilized and blocked for 30 minutes in 5% natural goat serum in PBS. Incubation with ab23631 (diluted 1/200) was for 1-4 hours at RT. DAPI was used for staining nuclei.
Top Left: Lingo1 staining
Top Right: Nestin staining
Bottom Left: Merge
