Anti-Oct-1 antibody (ab272867)
Key features and details
- Rabbit polyclonal to Oct-1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Oct-1 antibody
See all Oct-1 primary antibodies -
Description
Rabbit polyclonal to Oct-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human Oct-1. The exact sequence is proprietary. Center region.
Database link: P14859 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate, IHC-P: HeLa xenograft tissue and rat brain tissue. ICC/IF: HeLa cells. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Oct-1 antibody (ab272867)All lanes : Anti-Oct-1 antibody (ab272867) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (ab272867)Paraffin-embedded HeLa xenograft tissue stained for Oct-1 using ab272867 at 1/750 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody (ab272867)4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Oct-1 using ab272867 at 1/500 dilution in ICC/IF.
Red: Phalloidin, a cytoskeleton marker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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Immunoprecipitation - Anti-Oct-1 antibody (ab272867)Immunoprecipitation of Oct-1 protein from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using 5 μg of ab272867. ab272867 also used for WB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (ab272867)Paraffin-embedded rat brain tissue stained for Oct-1 protein using ab272867 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunoprecipitation - Anti-Oct-1 antibody (ab272867)Samples: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate:
A: Control with 2.5 μg of pre-immune rabbit IgG.
B: Immunoprecipitation of Oct-1 by 2.5 μg of ab272867.
7.5% SDS-PAGE
The immunoprecipitated Oct-1 protein was detected by ab272867 at 1:1000 dilution, followed by anti-rabbit IgG (HRP).
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Western blot - Anti-Oct-1 antibody (ab272867)All lanes : Anti-Oct-1 antibody (ab272867) at 1/200 dilution
Lane 1 : WT HeLa extracts
Lane 2 : Oct-1 KO HeLa extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG7.5% SDS-PAGE.