Anti-Smad2 (phospho T220) antibody (ab61066)
Key features and details
- Rabbit polyclonal to Smad2 (phospho T220)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 (phospho T220) antibody
See all Smad2 primary antibodies -
Description
Rabbit polyclonal to Smad2 (phospho T220) -
Host species
Rabbit -
Specificity
ab61066 detects endogenous levels of Smad2 only when phosphorylated at threonine 220. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).
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Positive control
- HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho T220) antibody (ab61066)Immunofluorescence analysis of HeLa cells, using ab61066 antibody at 1/500-1/1000 dilution with or without immunizing peptide.
