Anti-Histone H4 (propionyl K5) antibody (ab241252)
Key features and details
- Rabbit polyclonal to Histone H4 (propionyl K5)
- Suitable for: IP, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (propionyl K5) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (propionyl K5) -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (propionyl K5).
Database link: P62805 -
Positive control
- WB: HeLa, Jurkat and HEK-293T, treated with 10mM sodium propionate for 4hr, whole cell lysates. ICC: HeLa cells (treated with 10mM sodium propionate for 4hr). IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H4 (propionyl K5) antibody (ab241252)All lanes : Anti-Histone H4 (propionyl K5) antibody (ab241252) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 4 : HeLa whole cell lysate, untreated (-)
Lane 5 : Jurkat whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
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Immunoprecipitation - Anti-Histone H4 (propionyl K5) antibody (ab241252)Histone H4 (propionyl K5) was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab241252 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab241252 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
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Immunocytochemistry - Anti-Histone H4 (propionyl K5) antibody (ab241252)HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 10mM sodium propionate for 4hr) stained for Histone H4 (propionyl K5) using ab241252 at 1/100 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Western blot - Anti-Histone H4 (propionyl K5) antibody (ab241252)Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (4 x 106, treated with 10mM sodium propionate for 4h) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241252 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
